anti pan kac (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti pan kac
Anti Pan Kac, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2945 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pan+kac/Acetylated-Lysine+Antibody/10__1158_slash_0008___5472__can___25___3520-55-114-116
Average 96 stars, based on 2945 article reviews
Anti Pan Kac, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2945 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pan+kac/Acetylated-Lysine+Antibody/10__1158_slash_0008___5472__can___25___3520-55-114-116
Average 96 stars, based on 2945 article reviews
anti pan kac - by Bioz Stars,
2026-09
96/100 stars
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Membrane:Article Title: Lactate regulates pathological cardiac hypertrophy via histone lactylation modification. Article Snippet: The histones were extracted from the hearts of C57BL/6 mice and NMCMs following previously described methods.17,21 Target proteins were transferred to a PVDF membrane (MerckMillipore, USA) after being subjected to 10% SDS–PAGE. .. Afterwards, the membrane was obstructed using 5% skim milk at a temperature of 25°C for a duration of 1 h. This was then followed by an overnight incubation with primary antibodies against ANP (1:1000, 27426- 1- AP, Proteintech, China), BNP (1:1000, A2179, Abclonal, China), β- MHC (1:2000, A7564, Abclonal, China), LDHA (1:1500, A1146, Abclonal, China), LDHB (1:1500, A7625, Abclonal, China), P300 (1:1000, A1324, Abclonal, China), GAPDH (1:6000, AC001, Abclonal, China), Lactyl- Histone Antibody Sampler Kit (1:1000, PTM- 7093, PTMBiolab, China), Histone H3 (1:6000, A17562, Abclonal, China), H3K18ac (1:1000, A7257, Abclonal, China), H4K5ac (1:1000, A19525, Abclonal, China) and Incubation:Article Title: Lactate regulates pathological cardiac hypertrophy via histone lactylation modification. Article Snippet: The histones were extracted from the hearts of C57BL/6 mice and NMCMs following previously described methods.17,21 Target proteins were transferred to a PVDF membrane (MerckMillipore, USA) after being subjected to 10% SDS–PAGE. .. Afterwards, the membrane was obstructed using 5% skim milk at a temperature of 25°C for a duration of 1 h. This was then followed by an overnight incubation with primary antibodies against ANP (1:1000, 27426- 1- AP, Proteintech, China), BNP (1:1000, A2179, Abclonal, China), β- MHC (1:2000, A7564, Abclonal, China), LDHA (1:1500, A1146, Abclonal, China), LDHB (1:1500, A7625, Abclonal, China), P300 (1:1000, A1324, Abclonal, China), GAPDH (1:6000, AC001, Abclonal, China), Lactyl- Histone Antibody Sampler Kit (1:1000, PTM- 7093, PTMBiolab, China), Histone H3 (1:6000, A17562, Abclonal, China), H3K18ac (1:1000, A7257, Abclonal, China), H4K5ac (1:1000, A19525, Abclonal, China) and Aqueous Normal-phase Chromatography:Article Title: Lactate regulates pathological cardiac hypertrophy via histone lactylation modification. Article Snippet: The histones were extracted from the hearts of C57BL/6 mice and NMCMs following previously described methods.17,21 Target proteins were transferred to a PVDF membrane (MerckMillipore, USA) after being subjected to 10% SDS–PAGE. .. Afterwards, the membrane was obstructed using 5% skim milk at a temperature of 25°C for a duration of 1 h. This was then followed by an overnight incubation with primary antibodies against ANP (1:1000, 27426- 1- AP, Proteintech, China), BNP (1:1000, A2179, Abclonal, China), β- MHC (1:2000, A7564, Abclonal, China), LDHA (1:1500, A1146, Abclonal, China), LDHB (1:1500, A7625, Abclonal, China), P300 (1:1000, A1324, Abclonal, China), GAPDH (1:6000, AC001, Abclonal, China), Lactyl- Histone Antibody Sampler Kit (1:1000, PTM- 7093, PTMBiolab, China), Histone H3 (1:6000, A17562, Abclonal, China), H3K18ac (1:1000, A7257, Abclonal, China), H4K5ac (1:1000, A19525, Abclonal, China) and Immunopeptidomics:Article Title: Lactate regulates pathological cardiac hypertrophy via histone lactylation modification. Article Snippet: The histones were extracted from the hearts of C57BL/6 mice and NMCMs following previously described methods.17,21 Target proteins were transferred to a PVDF membrane (MerckMillipore, USA) after being subjected to 10% SDS–PAGE. .. Afterwards, the membrane was obstructed using 5% skim milk at a temperature of 25°C for a duration of 1 h. This was then followed by an overnight incubation with primary antibodies against ANP (1:1000, 27426- 1- AP, Proteintech, China), BNP (1:1000, A2179, Abclonal, China), β- MHC (1:2000, A7564, Abclonal, China), LDHA (1:1500, A1146, Abclonal, China), LDHB (1:1500, A7625, Abclonal, China), P300 (1:1000, A1324, Abclonal, China), GAPDH (1:6000, AC001, Abclonal, China), Lactyl- Histone Antibody Sampler Kit (1:1000, PTM- 7093, PTMBiolab, China), Histone H3 (1:6000, A17562, Abclonal, China), H3K18ac (1:1000, A7257, Abclonal, China), H4K5ac (1:1000, A19525, Abclonal, China) and Blocking Assay:Article Title: Spatial heterogeneity of lactylation: insights into gene expression, metabolism, and lactate transport in human embryonic stem cells Article Snippet: PVDF membranes were stained with 0.1% ponceau S in 5% acetic acid before being washed with 1× Tris-buffered saline with Tween-20 (TBST) at room temperature before being blocked in 5% milk (#9999; Cell Signaling Technology) or 5% bovine serum albumin (BSA) (#ALB005.100; BioShop) in 1× TBST for 60 min at room temperature ( ). .. Following blocking, membranes were rewashed with 1× TBST at room temperature before incubating with the following primary antibodies: 1:1000 pan Kla (#1401; PTM BIO); 1:16,000 H3K18la (#1406; PTM BIO), 1:20,000 Article Title: Spatial heterogeneity of lactylation: Insights into gene expression, metabolism, and lactate transport in human embryonic stem cells. Article Snippet: PVDF membranes were stained with 0.1% ponceau S in 5% Acetic Acid before being washed with 1x TBST at room temperature before being blocked in 5% milk (#9999; Cell Signaling) or 5% Bovine Serum Albumin (BSA) (#ALB005.100; BioShop) in 1X TBST for 60 min at room temperature (Table 1). .. Following blocking, membranes were rewashed with 1x TBST at room temperature before incubating with the following primary antibodies: 1:1000 pan Kla (#1401; PTM BIO); 1:16,000 H3K18la (#1406; PTM BIO), 1:20,000 Ubiquitin Proteomics:Article Title: BRD4 regulates m 6 A of ESPL1 mRNA via interaction with ALKBH5 to modulate breast cancer progression Article Snippet: .. The primary antibodies used in this study were ALKBH5 (Millipore, ABE547), BRD4 (CST, 13440S), METTL3 (CST, 86132S), METTL14 (CST, 51,104), FTO (Abcam, ab126605), GAPDH (Bioworld, AP0063), c-Myc (Thermo Fisher, MA1-980), ubiquitin (Abcam, ab7254), TRIM21(Abclonal, A1957), vimentin (CST, 5741S), fibronectin (FN, Santa, sc-8422), HDAC2 (ABclonal, A2084), Flag (Sigma, 7425/1804), |